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KMID : 0545120120220101446
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Journal of Microbiology and Biotechnology
2012 Volume.22 No. 10 p.1446 ~ p.1451
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A Study on the Electrochemical Synthesis of L-DOPA Using Oxidoreductase Enzymes: Optimization of an Electrochemical Process
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Siti Fauziyah Rahman
Sriramulu Gobikrishnan
Natarianto Indrawan
Park Seok-Hwan
Park Jae-Hee
Min Kyoung-Seon
Yoo Young-Je
Park Don-Hee
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Abstract
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Levodopa or L-3,4-dihydroxyphenylalanine (L-DOPA) is the precursor of the neurotransmitter dopamine. L-DOPA is a famous treatment for Parkinson’s disease symptoms. In this study, electroenzymatic synthesis of L-DOPA was performed in a three-electrode cell, comprising a Ag/AgCl reference electrode, a platinum wire auxiliary electrode, and a glassy carbon working electrode. L-DOPA had an oxidation peak at 376 mV and a reduction peak at -550 mV. The optimum conditions of pH, temperature, and amount of free tyrosinase enzyme were pH 7, 30oC, and 250 IU, respectively. The kinetic constant of the free tyrosinase enzyme was found for both cresolase and catacholase activity to be 0.25 and 0.4 mM, respectively. A cyclic voltammogram was used to investigate the electron transfer rate constant. The mean heterogeneous electron transfer rate (ke) was 5.8 × 10-4 cm/s. The results suggest that the electroenzymatic method could be an alternative way to produce L-DOPA without the use of a reducing agent such as ascorbic acid.
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KEYWORD
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L-DOPA, electrochemical synthesis, tyrosinase, Parkinson’s disease
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